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Resources
Please use the links on this page to access several resources provided by the Nuñez Lab
Reagents and Protocols
Cloning sgRNAs
This protocol is for cloning sgRNA oligonucleotides into sgRNA-expressing vectors, using BstXI and BlpI restriction enzymes from Jonathan Weissman's lab.
Choosing sgRNAs for CRISPRi and CRISPRoff
Coming soon
Epigenome editing assay by plasmid DNA and mRNA delivery
Please refer to the following manuscripts for delivering CRISPR epigenome editors by plasmid DNA or mRNA-based delivery.
CRISPRoff epigenome editing for programmable gene silencing in human cell lines and primary T cells (2025). Link here.
CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery (2025). Link here.
RENDER: Protein-based delivery of CRISPR epigenome editors using virus-like particles
Coming soon
Computational pipelines: EM-seq, RNA-seq, ATAC-seq
CRISPR screen protocol
Coming soon
Gene essentiality datasets
Coming soon
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