Resources

Please use the links on this page to access several resources provided by the Nuñez Lab

Reagents and Protocols

Cloning sgRNAs

This protocol is for cloning sgRNA oligonucleotides into sgRNA-expressing vectors, using BstXI and BlpI restriction enzymes from Jonathan Weissman's lab.

Click here for protocol

Choosing sgRNAs for CRISPRi and CRISPRoff

Coming soon

Epigenome editing assay by plasmid DNA and mRNA delivery

Please refer to the following manuscripts for delivering CRISPR epigenome editors by plasmid DNA or mRNA-based delivery.

CRISPRoff epigenome editing for programmable gene silencing in human cell lines and primary T cells (2025). Link here.

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery (2025). Link here.

RENDER: Protein-based delivery of CRISPR epigenome editors using virus-like particles

Coming soon

Computational pipelines: EM-seq, RNA-seq, ATAC-seq

Please visit our GitHub page for our computational pipelines.

https://github.com/Nunez-Lab

CRISPR screen protocol

Coming soon

Gene essentiality datasets

Coming soon